human ythdc1 recombinant protein  (Abnova)

Journal: Heliyon

Article Title: Endothelial downregulation of nuclear m6A reader YTHDC1 promotes pulmonary vascular remodeling in sugen hypoxia model of pulmonary hypertension

doi: 10.1016/j.heliyon.2024.e24963

Figure Lengend Snippet: N6-Methyladenosine regulators expression were dysregulated in PAECs. (A) m6A modulators were differently expressed in PAECs. (B–C) GO analysis of overlapped YTHDC1 target genes with the upregulated DEGs(B), genes in GO enriched pathways(C). (D–E) KEGG analysis of overlapped YTHDC1 target genes with the upregulated DEGs(D), genes in KEGG enriched pathways (E). (F) Hub networks based on genes in enriched pathways.

Article Snippet: HPAECs were seeded in 96 well plates at 1 × 10 4 cells/well and cultured for 24 h. After serum starved for 24 h, cells were incubated under hypoxia conditions (3 % Oxygen concentration) with human YTHDC1 recombinant protein (Abnova) for 24h.

Techniques: Expressing

Journal: Heliyon

Article Title: Endothelial downregulation of nuclear m6A reader YTHDC1 promotes pulmonary vascular remodeling in sugen hypoxia model of pulmonary hypertension

doi: 10.1016/j.heliyon.2024.e24963

Figure Lengend Snippet: YTHDC1 was decreased in PAECs isolated from SuHx PH mouse models. (A) The heatmap of differentially expressed genes in the datasets. (B) Different expressions of m6A regulators. (C) Expressions of RBM15b, YTHDC1, ALKBH5 and WTAP in Control and SuHx group. (D) KEGG analysis of m6A target genes overlapped with upregulated genes. (E–F) Genes involved in pathways were presented by KEGG（E）and GO（F）analysis.

Article Snippet: HPAECs were seeded in 96 well plates at 1 × 10 4 cells/well and cultured for 24 h. After serum starved for 24 h, cells were incubated under hypoxia conditions (3 % Oxygen concentration) with human YTHDC1 recombinant protein (Abnova) for 24h.

Techniques: Isolation, Control

Journal: Heliyon

Article Title: Endothelial downregulation of nuclear m6A reader YTHDC1 promotes pulmonary vascular remodeling in sugen hypoxia model of pulmonary hypertension

doi: 10.1016/j.heliyon.2024.e24963

Figure Lengend Snippet: Endothelial downregulation of YTHDC1 promotes PAECs proliferation. (A) YTHDC1 was downregulated in lung samples and CD31 positive cells of SuHx PH model (right) compared with controls(left), determined by IHC. (B) Proliferation of HPAECs was detected by CCK8 under hypoxia conditions. (C) YTHDC1 was decreased in HPAECs under hypoxia conditions. (D) The effect of YTHDC1 recombinant protein on HPAEC proliferation was detected by CCK8 (*p＜0.05,**p＜0.01).

Article Snippet: HPAECs were seeded in 96 well plates at 1 × 10 4 cells/well and cultured for 24 h. After serum starved for 24 h, cells were incubated under hypoxia conditions (3 % Oxygen concentration) with human YTHDC1 recombinant protein (Abnova) for 24h.

Techniques: Recombinant

Journal: Heliyon

Article Title: Endothelial downregulation of nuclear m6A reader YTHDC1 promotes pulmonary vascular remodeling in sugen hypoxia model of pulmonary hypertension

doi: 10.1016/j.heliyon.2024.e24963

Figure Lengend Snippet: YTHDC1 participated in regulating PAECs through cellular interaction (A) Increasing receptor-ligand pairs regulated by YTHDC1 were shown. (B) Decreasing receptor-ligand pairs regulated by YTHDC1 were shown.

Article Snippet: HPAECs were seeded in 96 well plates at 1 × 10 4 cells/well and cultured for 24 h. After serum starved for 24 h, cells were incubated under hypoxia conditions (3 % Oxygen concentration) with human YTHDC1 recombinant protein (Abnova) for 24h.

Techniques:

Journal: Heliyon

Article Title: Endothelial downregulation of nuclear m6A reader YTHDC1 promotes pulmonary vascular remodeling in sugen hypoxia model of pulmonary hypertension

doi: 10.1016/j.heliyon.2024.e24963

Figure Lengend Snippet: YTHDC1-lncRNA/miRNA/mRNA interaction promote PAECs dysfunction. (A) Predicted TFs regulating YTHDC1. (B) lncRNA-miRNA-mRNA ceRNA network in PAECs. Yellow, orange nodes represent lncRNAs, miRNAs. Purple and blue nodes represent mRNAs, the later were YTHDC1 target genes. (C) PPI network of the mRNAs in the ceRNA network.

Article Snippet: HPAECs were seeded in 96 well plates at 1 × 10 4 cells/well and cultured for 24 h. After serum starved for 24 h, cells were incubated under hypoxia conditions (3 % Oxygen concentration) with human YTHDC1 recombinant protein (Abnova) for 24h.

Techniques:

Journal: Heliyon

Article Title: Endothelial downregulation of nuclear m6A reader YTHDC1 promotes pulmonary vascular remodeling in sugen hypoxia model of pulmonary hypertension

doi: 10.1016/j.heliyon.2024.e24963

Figure Lengend Snippet: The top 5 lncRNAs predicted via YTHDC1 revolving in interaction with miRNAs.

Article Snippet: HPAECs were seeded in 96 well plates at 1 × 10 4 cells/well and cultured for 24 h. After serum starved for 24 h, cells were incubated under hypoxia conditions (3 % Oxygen concentration) with human YTHDC1 recombinant protein (Abnova) for 24h.

Techniques: